Assessment of copper and zinc status of farm horses and training thoroughbreds in south-east Queensland   总被引：2，自引：0，他引：2  

DE AUER  JC NG  AA SEAWRIGHT 《Australian veterinary journal》1988,65(10):317-320

The copper and zinc concentrations in the blood of stabled thoroughbred horses and in Australian Stock Horses mares at pasture, either late pregnant or lactating were determined by an atomic absorption spectroscopic method. The plasma concentration of the trace elements in these apparently normal horses were generally below the "normal" range. The plasma copper, caeruloplasmin copper, whole blood copper and plasma zinc concentrations in the stabled thoroughbreds were 0.76 +/- 0.19 micrograms/ml (n = 82), 0.56 +/- 0.14 micrograms/ml (n = 83), 0.75 +/- 0.18 micrograms/ml (n = 82) and 0.47 +/- 0.09 micrograms/ml (n = 83) respectively. The plasma copper and zinc concentrations of all the brood mares at pasture (pregnant and lactating) were 0.56 +/- 0.20 micrograms/ml and 0.47 +/- 0.11 micrograms/ml (n = 30). The plasma copper concentration of the pregnant group of mares (0.64 +/- 0.18 micrograms/ml; (n = 14) was greater than that of the lactating mares (0.49 +/- 0.21; (n = 16). Variation in the plasma copper concentration was also identified between stabled and farm horses, between horses of different stables and between horses of different ages. The proportion of plasma copper bound to caeruloplasmin was 73 +/- 11.8%. These low concentrations of copper and zinc in the plasma of apparently normal horses are of clinical significance since recent evidence has indicated that copper deficiency appears to promote the development of skeletal abnormalities in foals. An alternative to the use of a single plasma sample to identify the copper or zinc deficient horse was discussed.  相似文献   

Dietary inclusion of tomato pomace improves meat oxidative stability of young pigs          下载免费PDF全文

C. S. Correia  C. M. Alfaia  M. S. Madeira  P. A. Lopes  T. J. S. Matos  L. F. Cunha  J. A. M. Prates  J. P. B. Freire 《Journal of animal physiology and animal nutrition》2017,101(6):1215-1226

The aim of this study was to assess the nutritional effect of tomato pomace, in combination with two distinct fat sources (lard or soya bean oil), on longissimus lumborum muscle, subcutaneous fat and liver of young pigs. Forty male pigs of 4 weeks old were randomly assigned to four dietary treatments using two fibre sources (wheat bran or 5.0% tomato pomace) and two fat sources (lard or soya bean oil), in a 2 × 2 factorial design for a 5‐week trial. Tomato pomace did not improve colour, fatty acid profile, cholesterol content or lipid oxidation, nor did it yield detectable levels in lycopene or β‐carotene in the meat (p > 0.05). However, a positive increase in α‐tocopherol content of meat and liver was observed in pigs fed tomato pomace (p < 0.05). Compared with lard, soya bean oil increased MUFA and PUFA proportions and decreased the percentage of SFA (p < 0.05). Our results indicate that 5.0% of tomato pomace increases pork oxidative stability and that 5.0% of soya bean oil, compared with lard, improves its nutritional quality.  相似文献   

Assessment of serological tests for the diagnosis of canine visceral leishmaniasis     

Denise Amaro da Silva  Maria de Fátima Madeira  Tuanne Rotti Abrantes  Carlos José de Lima Barbosa Filho  Fabiano Borges Figueiredo 《Veterinary journal (London, England : 1997)》2013,195(2):252-253

An immunoenzymatic assay (ELISA), an indirect immunofluorescence antibody test (IFAT) with different antigens (ELISA-Leishmania chagasi, ELISA-L. major-like, IFAT-L. chagasi and IFAT-L. major-like), and an immunochromatographic test were assessed for the diagnosis of canine visceral leishmaniasis (CVL). Serum samples from 144 dogs from an endemic area for visceral leishmaniasis in the municipality of Rio de Janeiro were tested. The sensitivities of the serological tests were 93%, 100%, 73%, 60% and 93%, with specificities of 87%, 92%, 77%, 96% and 92% for the ELISA-L. major-like, ELISA-L. chagasi, IFAT-L. major-like, IFAT-L. chagasi and the immuno chromatographic test, respectively. ELISA-L. chagasi was the best test for the diagnosis of CVL, but the immunochromatographic test could be a useful alternative as it offers simple and rapid diagnosis without the need for a specialized laboratory.  相似文献   

Use of ELISA employing Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi antigens for the detection of IgG and IgG1 and IgG2 subclasses in the diagnosis of American tegumentary leishmaniasis in dogs     

Ribeiro FC  de O Schubach A  Mouta-Confort E  Schubach TM  de Fátima Madeira M  Marzochi MC 《Veterinary parasitology》2007,148(3-4):200-206

American tegumentary leishmaniasis (ATL) shows a reduced humoral response in dogs and levels of specific antibodies may therefore not be detected by indirect immunofluorescence. Although the sensitivity of enzyme-linked immunosorbent assay (ELISA) is higher than that of indirect immunofluorescence, the best antigen for the diagnosis of ATL in dogs has not been defined. The detection of IgG subclasses represents an alternative to increase the efficiency of the serological diagnosis. In Rio de Janeiro, sporotrichosis is the main differential diagnosis of ATL in dogs, and a sensitive, specific and little invasive method that permits the discrimination of the two diseases is desired. In the present study, 69 serum samples, 34 obtained from dogs with ATL and 35 from dogs with sporotrichosis, all of them with a confirmed etiological diagnosis, were tested. The samples were analyzed by ELISA using Leishmania (Viannia) braziliensis and L. (L.) chagasi antigens for the detection of anti-Leishmania IgG, IgG1 and IgG2. The use of L. (V.) braziliensis antigens for the detection of IgG and IgG2 yielded the best results. Using L. (L.) chagasi antigen, the sensitivity and specificity for the detection of IgG were 82.4% and 100%, respectively, whereas both sensitivity and specificity were 97.1% with the L. (V.) braziliensis antigen. No improvement in the performance of the test was observed when IgG2 was analyzed separately. The IgG1 assays presented low accuracy, irrespective of the antigen used: sensitivity and specificity of 58.8% and 60% for L. (V.) braziliensis and of 64.7% and 77.1% for L. (L.) chagasi, respectively. The present results suggest that IgG ELISA using the L. (V.) braziliensis shows the best performance for the diagnosis of ATL, permitting the discrimination between cases of ATL and sporotrichosis in dogs.  相似文献   

A two-enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets     

Diogo Coelho  Paula A. Lopes  Vânia Cardoso  Patrícia Ponte  Joana Brás  Marta S. Madeira  Cristina M. Alfaia  Narcisa M. Bandarra  Carlos M. G. A. Fontes  José A. M. Prates 《Journal of animal physiology and animal nutrition》2020,104(1):310-321

The main goal of this study was to test a rational combination of pre-selected carbohydrate-active enzymes (CAZymes) and sulphatases, individually or in combination, in order to evaluate its capacity to disrupt Arthrospira platensis cell wall, allowing the release of its valuable nutritional bioactive compounds. By the end, a two-enzyme constituted mixture (Mix), composed by a lysozyme and a α-amylase, was incubated with A. platensis suspension. The microalga cell wall disruption was evaluated through the amount of reducing sugars released from the cell wall complemented with the oligosaccharide profile by HPLC. An increase of the amount of reducing sugars up to 2.42 g/L in microalgae treated with the Mix relative to no treatment (p < .05), as well as a 7-fold increase of oligosaccharides amount (p < .001), were obtained. With resort of fluorescence microscopy, a 36% reduction of fluorescence intensity (p < .001) was observed using Calcofluor White staining. In the supernatant, the Mix caused a 1.34-fold increase in protein content (p = .018) relative to the control. Similarly, n-6 polyunsaturated fatty acids (PUFA) (p = .007), in particular 18:2n-6 (p = .016), monounsaturated fatty acids (MUFA) (p = .049) and chlorophyll a (p = .025) contents were higher in the supernatant of microalgae treated with the enzyme mixture in relation to the control. Taken together, these results point towards the disclosure of a novel two-enzyme mixture able to partial degrade A. platensis cell wall, improving its nutrients bioavailability for monogastric diets with the cost-effective advantage use of microalgae in animal feed industry.  相似文献   

Palm oil-laden spent bleaching clay as a substitute for marine fish oil in the diets of Nile tilapia, Oreochromis niloticus     

W.-K. NG  C.-B. KOH  & Z.B. DIN 《Aquaculture Nutrition》2006,12(6):459-468

A 8‐week feeding trial was conducted to determine the effect of substituting fish oil with palm oil‐laden spent bleaching clay (SBC), a by‐product from crude palm oil (CPO) refining, on growth, feed utilization, fatty acid composition and heavy metal accumulation in the muscle of Nile tilapia, Oreochromis niloticus. Four isonitrogenous and isolipidic practical diets were formulated to contain 0, 100, 200 or 300 g kg^?1 SBC. Growth performance of Nile tilapia was significantly better in fish fed the 100 g kg^?1 SBC diet compared with fish fed the 0, 200 or 300 g kg^?1 SBC diet. Growth and feed utilization efficiency of fish fed 200 or 300 g kg^?1 SBC were similar to fish fed the control diet without added SBC. Whole‐body composition, body‐organ indices and haematocrit of tilapia were not affected by dietary treatments. Fatty acid compositions in the muscle lipid of Nile tilapia were strongly influenced by dietary treatments with progressively elevated levels of total saturates and n‐6 PUFA because of the dietary influence of these fatty acids from residual CPO adsorbed onto SBC. A gradual decrease in total n‐3 PUFA concentrations were also observed with the ratio of n‐3 to n‐6 fatty acids in muscle lipids decreasing from 4.75 to 4.41, 3.23 or 2.37 after 8 weeks on the 0, 100, 200 or 300 g kg^?1 SBC diet, respectively. The arsenic, cadmium and lead concentrations in the experimental diets increased with increasing dietary levels of SBC but the concentrations of these heavy metals in the whole body and bone of Nile tilapia were not significantly different among fish fed the various diets. The present 8‐week study showed that in fishmeal‐based diets for Nile tilapia, palm oil‐laden SBC can totally replace added fish oil. The use of this presently discarded waste product from palm oil refining in tilapia diets will greatly contribute to reducing the impact of rising feed costs in the culture of tilapia in many tropical countries. Other potential benefits may include acting as a feed binder, removal of mycotoxins in fish feeds as well as adsorbing toxic substances present in the culture water.  相似文献   

The use of hand-held terminals and micro-computers for the recording and analysis of potato cyst nematode counts     

Linda Farrer  C. M. NG Stephen 《Euphytica》1982,31(2):507-510

Summary An automated system of data capture and summarisation is described. Although it is of widespread applicability, it is illustrated by a specific example involving the screening of potato tubers for resistance to potato cyst nematode.  相似文献   

实时实地氮肥管理对水稻产量和氮素利用率的影响   总被引：117，自引：12，他引：117  

刘立军  桑大志  刘翠莲  王志琴  杨建昌  朱庆森 《中国农业科学》2003,36(12):1456-1461

 以籼型三系杂交稻汕优 63为材料 ,对不同氮肥管理的农艺和经济表现进行了评价。结果表明 ,未施氮肥 (空白区 )的产量为 6.8～ 7.4t·ha- 1,表明试验田背景氮颇高。与农民习惯施肥法 (氮肥 2 40kg·ha- 1)相比 ,改良的农民施肥法 (农民习惯施氮量的 70 % )、实时氮肥管理 (依据叶绿素仪测定值进行的施肥方法 )和实地氮肥管理(施氮时期、施氮量和叶绿素仪测定值相结合的施肥方法 )分别增产 9.2 %～ 10 .3 %、3 .3 %～ 7.0 %和 8.9%～ 9.3 % ,氮肥农学利用率分别提高了 110 .5%～ 13 5.6%、2 0 4.3 %～ 2 97.0 %和 2 0 0 .9%～ 2 76.4%。以上结果表明 ,在产量不降低甚至有所增加的前提下 ,实时、实地氮肥管理可以减少氮肥施用量 ,较大幅度地提高水稻氮肥利用率。另外 ,实时、实地氮肥管理还可以降低稻米的垩白率和垩白度 ,改善稻米的外观品质  相似文献   

北京市城区近郊区生活垃圾成分调查及可农用性分析     

刘克锋  石爱平  王红利  刘悦秋  刘彩玲  邢汝明 《农业环境科学学报》2002,21(3):232-236

对北京市城区和近郊区的新鲜城市生活垃圾进行了分类调查和理化性质测定。结果表明:(1)新鲜生活垃圾可农用部分占84.04%,冬季可农用部分所占比率高;(2)垃圾肥料有机质含量在12.50%—19.70%范围,全氮在0.53%—0.87%范围;(3)垃圾肥料重金属含量和残毒含量符合国家卫生标准和城市垃圾农用控制标准。建议垃圾肥料与处理过的禽畜粪便混合,可生产出优质有机肥料。  相似文献   

Single-Electron Transport in Ropes of Carbon Nanotubes     

M Bockrath  DH Cobden  PL McEuen  NG Chopra  A Zettl  A Thess  RE Smalley 《Science (New York, N.Y.)》1997,275(5308):1922-1925

The electrical properties of individual bundles, or "ropes," of single-walled carbon nanotubes have been measured. Below about 10 kelvin, the low-bias conductance was suppressed for voltages less than a few millivolts. In addition, dramatic peaks were observed in the conductance as a function of a gate voltage that modulated the number of electrons in the rope. These results are interpreted in terms of single-electron charging and resonant tunneling through the quantized energy levels of the nanotubes composing the rope.  相似文献